443143416pLVTH
- 產(chǎn)品貨號GS-1411
- 銷售價格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類型高
- 載體抗性Ampicillin (氨芐青霉素)
- 載體大小11086 bp
- 啟動子EF-1 alpha
- 5'測序引物EF-1a Forward: TCAAGCCTCAGACAGTGGTTC
- 備注說明載體使用Stbl3感受態(tài)細(xì)胞擴(kuò)增質(zhì)粒，可以減少載體基因重組幾率。該載體已經(jīng)被pLVTHM載體替代，pLVTHM載體允許shRNA的定向克隆。
載體說明
This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA. For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning. Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M et al. (J Virol. 2003 Aug . 77(16):8957-61.
