443143416pCMV-BD
- 產(chǎn)品貨號(hào)GS-1656
- 銷售價(jià)格￥ 680
- 包裝規(guī)格2μg質(zhì)?；?.5ml甘油菌
- 表達(dá)類型高
- 載體抗性Kanamycin (卡那霉素)
- 篩選標(biāo)記Neomycin (新霉素)
- 載體大小4730 bp
- 啟動(dòng)子CMV
The pCMV-BD and pCMV-AD vectors are designed for the construction and expression of gene fusions with the GAL4 DNA binding domain and NF-κB transcriptional activation domain, respectively. The pCMV-BD vector contains DNA encoding amino acids 1–147 of the GAL4 gene (DNA binding domain)2 and unique 3′ cloning sites. It is used for the construction of bait plasmids containing a DNA insert encoding a bait protein. The pCMV-AD vector contains DNA encoding the nuclear localization sequence (NLS) from SV40 large T-antigen (PKKKRKV), amino acids 364–550 of the mouse NF-κB gene (transcriptional activation domain)3 and unique 3′ cloning sites. It is used for constructing a target plasmid containing a DNA insert encoding the interacting proteins. Both pCMV-BD and pCMV-AD contain the pUC origin for replication in E. coli. The pCMV-BD vector contains the kanamycin-resistance gene and the pCMV-AD vector contains the ampicillin-resistance gene. The constitutively-active cytomegalovirus (CMV) promoter governs the expression of the bait and target proteins in the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) provide the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells.
0001 ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA
0061 GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG
0121 CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG
0181 ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA
0241 TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC
0301 CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC
0361 TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC
0421 ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA
0481 TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG
0541 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA
0601 GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTGATCT
0661 AAGCCTCCTG AAAGATGAAG CTACTGTCTT CTATCGAACA AGCATGCGAT ATTTGCCGAC
0721 TTAAAAAGCT CAAGTGCTCC AAAGAAAAAC CGAAGTGCGC CAAGTGTCTG AAGAACAACT
0781 GGGAGTGTCG CTACTCTCCC AAAACCAAAA GGTCTCCGCT GACTAGGGCA CATCTGACAG
0841 AAGTGGAATC AAGGCTAGAA AGACTGGAAC AGCTATTTCT ACTGATTTTT CCTCGAGAAG
0901 ACCTTGACAT GATTTTGAAA ATGGATTCTT TACAGGATAT AAAAGCATTG TTAACAGGAT
0961 TATTTGTACA AGATAATGTG AATAAAGATG CCGTCACAGA TAGATTGGCT TCAGTGGAGA
1021 CTGATATGCC TCTAACATTG AGACAGCATA GAATAAGTGC GACATCATCA TCGGAAGAGA
1081 GTAGTAACAA AGGTCAAAGA CAGTTGACTG TATCGCCGGA ATTGGGATCC GCTAGCCCGG
1141 GCGAATTCGG AAGCTTGCGG CCGCGTCGAC CTCTAGACTG CAGCTCGACC TCGAGGGGGG
1201 GCCCGGTACC AGGTAAGTGT ACCCAATTCG CCCTATAGTG AGTCGTATTA CAATTCACTC
1261 GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGAGATCCAA TTTTTAAGTG
1321 TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCAC AGTCCCAAGG
1381 CTCATTTCAG GCCCCTCAGT CCTCACAGTC TGTTCATGAT CATAATCAGC CATACCACAT
1441 TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA
1501 AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
1561 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
1621 TGTCCAAACT CATCAATGTA TCTTAACGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA
1681 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA
1741 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC
1801 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG
1861 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT
1921 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG
1981 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA
2041 AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG
2101 GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC
2161 TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA
2221 TATTGAAAAA GGAAGAATCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG
2281 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT
2341 AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA
2401 TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA
2461 CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG
2521 AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG
2581 GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA
2641 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC
2701 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG
2761 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG
2821 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
2881 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG
2941 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG
3001 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA
3061 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAACATCAG
3121 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT
3181 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
3241 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
3301 GCTACCCGTG ATATTGCTGA AGAACTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
3361 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
3421 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC
3481 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG
3541 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA
3601 GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG
3661 CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC
3721 GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC
3781 GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG
3841 CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG
3901 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
3961 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
4021 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
4081 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
4141 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT
4201 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
4261 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
4321 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
4381 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
4441 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
4501 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
4561 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
4621 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
4681 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC